Blood – Special Tests

Antiglobulin (Coomb’s) Cross–matching
The antiglobulin cross–match, if compatible, is a high degree of assurance that the transfused blood will be successfully accepted by the patient.

Tests to be Performed and Criteria for Blood Transfusion
Most important tests performed are:

Blood grouping and Rh factor.
Antibody detection by indirect antiglobulin (Coomb’s) test for a typical, incomplete antibodies.

Other tests performed on collected blood are

Hepatitis – B surface antigen.
AIDS antibodies.
Syphilis (VDRL) test.
Malarial parasites.

Criteria accepted for selection of donor

The age should be above 18 – 60 years.
Hemoglobin should not be less than 12.5 g/dl.
Interval of 16 weeks should be there between two donations.
Pulse rate should be regular and between to 78 – 100/min.
Accepted Blood Pressure.
- Systolic: between 90 – 150 mm Hg.
- Diastolic: between 50 – 100 mm Hg.
Body temperature of donor should be normal.
Absence of any chronic disease.

The donor is rejected if

There is a history of viral hepatitis or AIDS.
There is a history of malaria for the last three years.
If the patient has received immunization against rabies, mumps, rubella, small pox within two months.
If the donor has undergone dental surgery within 72 hours.

Semi–quantitative determination of Anti–D Antibody Titer
This is useful to defect hemolytic disease of the newborn.

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